3VYY
Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase A (DHX9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.007 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.252, 78.529, 112.242 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 37.010 - 2.900 |
| R-factor | 0.22097 |
| Rwork | 0.219 |
| R-free | 0.26175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1whq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 9511 | |
| <I/σ(I)> | 27.7 | 5.8 |
| Completeness [%] | 99.5 | 94.7 |
| Redundancy | 3.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, CaAc2, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
