3VYU

Crystal structure of the HypC-HypD-HypE complex (form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-24
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	114.677, 121.461, 67.354
Unit cell angles	90.00, 97.20, 90.00

Refinement procedure

Resolution	36.810 - 2.750
R-factor	0.219
Rwork	0.219
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z1c 2z1d 2z1e
RMSD bond length	0.012
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.750	2.750
Number of reflections	21147
<I/σ(I)>	19.8	1.9
Completeness [%]	88.7	55.7
Redundancy	3.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7	293	0.1M Tris-HCl, 12-16%(w/v) PEG8000, 2% ethylene glycol, pH 8.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K