3VW2
Crystal Strucuture of The Berberine-bound Form of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-23 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9000 |
Spacegroup name | P 1 |
Unit cell lengths | 43.817, 54.623, 92.291 |
Unit cell angles | 104.97, 97.74, 90.04 |
Refinement procedure
Resolution | 41.290 - 2.340 |
R-factor | 0.2266 |
Rwork | 0.225 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.746 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
High resolution limit [Å] | 2.340 | 6.370 | 2.350 |
Rmerge | 0.075 | 0.043 | 0.410 |
Number of reflections | 32813 | ||
<I/σ(I)> | 11.3 | ||
Completeness [%] | 95.1 | 85 | 90.9 |
Redundancy | 3.2 | 2.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES, 20mM NaPi, 75mM NaCl, 0.2M Ammonium sulfate, 2mM DTT, 20% PEG 6000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |