3VVX

Crystal Strucuture of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-27
Detector	RAYONIX MX225HE
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	39.792, 77.594, 120.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.920 - 2.050
R-factor	0.2363
Rwork	0.234
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.536
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.055	0.023	0.299
Number of reflections	24191
<I/σ(I)>	21.9
Completeness [%]	99.9	99.2	100
Redundancy	26	20.4	28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM MES, 20mM Tris-HCl, 75mM KCl, 2mM DTT, 5.88% PEG 20000, 50% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K