3VU4
Crystal structure of Kluyvelomyces marxianus Hsv2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-05-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 140.270, 140.270, 251.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.980 - 2.600 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.25200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.062 | 0.556 |
Number of reflections | 45676 | |
<I/σ(I)> | 17.4 | |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M AcOH, 1.2M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
2 |