3VU3

Crystal structure of the Hfq and catalase HPII complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-16
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	I 2 2 2
Unit cell lengths	136.432, 159.009, 167.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.850
R-factor	0.1989
Rwork	0.196
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qhs 1gge
RMSD bond length	0.013
RMSD bond angle	1.891
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.850	2.850
Number of reflections	40349
Completeness [%]	87.7	89.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1M Tris-HCl, 0.18M NaCl, 10% PEG4000, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K