3VU2
Structure of the Starch Branching Enzyme I (BEI) complexed with maltopentaose from Oryza sativa L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.810, 66.560, 169.625 |
Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
Resolution | 37.220 - 2.230 |
R-factor | 0.22908 |
Rwork | 0.226 |
R-free | 0.28641 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.016 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 72156 |
Completeness [%] | 88.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |