3VU1
Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (PhAglB-L, O74088_PYRHO) from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.467, 94.844, 186.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.1735 |
| Rwork | 0.171 |
| R-free | 0.21469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zag |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.294 |
| Number of reflections | 41657 | |
| <I/σ(I)> | 22.81 | 4 |
| Completeness [%] | 99.3 | 89.8 |
| Redundancy | 6.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15%(w/v) PEG3350, 0.2M sodium citrate, 0.1M bis-Tris propane-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
