3VTX
Crystal structure of MamA protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.939 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.457, 77.548, 77.963 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.980 - 1.750 |
R-factor | 0.1637 |
Rwork | 0.162 |
R-free | 0.20040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.027 |
RMSD bond angle | 2.024 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.980 | 1.791 |
High resolution limit [Å] | 1.745 | 1.740 |
Number of reflections | 47254 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vappor diffusion, sitting drop | 4.4 | 293 | 0.1M BICINE pH 9, 2M MgCl, Vappor diffusion, sitting drop, temperature 293K |