3VTN
The crystal structure of the C-terminal domain of Mu phage central spike - Pt derivative for MAD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0717, 1.0721, 1.0539 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 47.171, 47.171, 72.114 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.060 - 1.750 |
| R-factor | 0.27436 |
| Rwork | 0.273 |
| R-free | 0.30418 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.060 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 9825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 21% PEG 8000, 0.1M acetate pH 4.5, 0.2M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
