3VSS
Microbacterium saccharophilum K-1 beta-fructofuranosidase catalytic domain complexed with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.416, 71.979, 58.811 |
| Unit cell angles | 90.00, 95.09, 90.00 |
Refinement procedure
| Resolution | 39.560 - 1.970 |
| R-factor | 0.17407 |
| Rwork | 0.169 |
| R-free | 0.21796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vsr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.077 | 0.331 |
| Number of reflections | 33971 | |
| <I/σ(I)> | 25.4 | 4.6 |
| Completeness [%] | 98.5 | 96.1 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM sodium acetate pH 5.5, 22.5% polyethylene glycol 8000, 300mM glycine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
