3VSE
Crystal structure of methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.485, 91.573, 102.631 |
| Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
| Resolution | 34.126 - 2.099 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: dev_1021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.099 | 2.100 |
| Number of reflections | 103003 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 100mM sodium acetate pH4.5, 40 %(v/v) ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
