3VRC
Crystal structure of cytochrome c' from Thermochromatium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.310, 57.189, 54.225 |
| Unit cell angles | 90.00, 94.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.000 |
| R-factor | 0.1304 |
| Rwork | 0.129 |
| R-free | 0.15340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bbh |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.040 | 0.210 |
| Number of reflections | 127655 | |
| <I/σ(I)> | 42.6 | 2.6 |
| Completeness [%] | 94.3 | 70.2 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 30% PEG 400, 0.1M cadmium chloride, 0.1M sodium acetate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
