3VR2
Crystal structure of nucleotide-free A3B3 complex from Enterococcus hirae V-ATPase [eA3B3]
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 121.800, 121.500, 128.260 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 44.180 - 2.800 |
R-factor | 0.20061 |
Rwork | 0.198 |
R-free | 0.24293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gqb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.333 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.180 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.068 | 0.349 |
Number of reflections | 91463 | |
<I/σ(I)> | 11.6 | 3.5 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 24% PEG 3350, 0.1M Tris, 0.2M Ammonium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |