3VR1
Crystal structure analysis of the translation factor RF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.202, 83.461, 114.245 |
| Unit cell angles | 72.98, 74.18, 89.78 |
Refinement procedure
| Resolution | 41.600 - 3.000 |
| R-factor | 0.22025 |
| Rwork | 0.218 |
| R-free | 0.25875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vqt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 6.520 | 3.020 |
| Rmerge | 0.078 | ||
| Number of reflections | 52309 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 96.6 | 98.8 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% PEG 8000, 0.3M NaCl, 10mM alanine, 10mM arginine, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
