3VPZ
Crystal structure of glucokinase from Antarctic psychrotroph at 1.69A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SAGA-LS BEAMLINE BL07 |
| Synchrotron site | SAGA-LS |
| Beamline | BL07 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-31 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.610, 98.610, 59.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.560 - 1.690 |
| R-factor | 0.20379 |
| Rwork | 0.201 |
| R-free | 0.24801 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q18 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.783 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.390 | |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.075 | |
| Number of reflections | 36190 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 95.8 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M Trimethylamine N-oxide dihydrate, 20% PEGMME 2000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
