3VPN

Crystal structure of human ribonucleotide reductase subunit M2 (hRRM2) mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-04
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	109.082, 109.082, 176.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.340 - 2.250
R-factor	0.21335
Rwork	0.212
R-free	0.24671
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3olj
RMSD bond length	0.012
RMSD bond angle	1.137
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.250	4.850	2.250
Rmerge	0.074	0.033	0.692
Number of reflections	51165
<I/σ(I)>	10.5
Completeness [%]	99.9	99.5	100
Redundancy	9.6	9.2	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50mM Tris-HCl, 0.1mM KCl, pH 7.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP