3VPM

Crystal structure of human ribonucleotide reductase subunit M2 (hRRM2) mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-05-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97906
Spacegroup name	P 43 21 2
Unit cell lengths	108.998, 108.998, 174.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.630 - 2.700
R-factor	0.20944
Rwork	0.207
R-free	0.24899
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3olj
RMSD bond length	0.010
RMSD bond angle	1.168
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.100	0.042	0.700
Number of reflections	29863
<I/σ(I)>	10.4
Completeness [%]	99.5	99.4	99.8
Redundancy	13.6	13.4	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50mM Tris-HCl, 0.1mM KCl, pH 7.5, temperature 293K, VAPOR DIFFUSION, HANGING DROP