3VPJ
crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa in complex with immune protein Tsi1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-11-27 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 97.000, 97.000, 292.171 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.970 - 2.500 |
R-factor | 0.2095 |
Rwork | 0.207 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.059 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.610 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.067 | 0.387 |
Number of reflections | 56166 | |
<I/σ(I)> | 21.6 | 5.6 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 9.2 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 20% PEG 4000, 20% isopropanol, 0.1M sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |