3VPI
Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-11-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.450, 63.640, 73.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.610 - 1.500 |
R-factor | 0.1657 |
Rwork | 0.165 |
R-free | 0.18340 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.011 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.610 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.080 | 0.266 |
Number of reflections | 29544 | |
<I/σ(I)> | 14.7 | 4.8 |
Completeness [%] | 99.4 | 96 |
Redundancy | 6.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 1.8M sodium formate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 1.8M sodium formate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |