3VPG
L-lactate dehydrogenase from Thermus caldophilus GK24
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.240, 135.073, 85.877 |
Unit cell angles | 90.00, 94.44, 90.00 |
Refinement procedure
Resolution | 29.190 - 1.800 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ldb |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 111821 | |
Completeness [%] | 98.2 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2-(N-monorpholino)ethanesulfonic acid, polyethyleneglycol 6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |