3VP8
Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.170, 43.582, 70.525 |
Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
Resolution | 34.585 - 1.910 |
R-factor | 0.2471 |
Rwork | 0.244 |
R-free | 0.29840 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.852 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.600 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 25047 | |
Completeness [%] | 92.9 | 92.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 293 | 10%(v/v) 2-propanol, 0.1M phosphate-citrate, 0.2M Li2SO4, pH 4.2, VAPOR DIFFUSION, temperature 293K |