3VP7

Crystal structure of the beta-alpha repeated, autophagy-specific (BARA) domain of Vps30/Atg6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9670
Spacegroup name	P 41 21 2
Unit cell lengths	59.959, 59.959, 113.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 2.300
R-factor	0.209
Rwork	0.209
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.020	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.074	0.325
Number of reflections	9844
<I/σ(I)>	12.8
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	2.5M sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K