3VOZ

Crystal structure of human glutaminase in complex with BPTES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-28
Wavelength(s)	1.000
Spacegroup name	I 41 2 2
Unit cell lengths	139.542, 139.542, 156.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.400
Rwork	0.209
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3czd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	30507
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.8M Lithium sulfate, 100mM Bis-tris-propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K