3VOY

Crystal structure of human glutaminase in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	139.598, 139.598, 156.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.2002
Rwork	0.200
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3czd
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	38287
Completeness [%]	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.4M Lithium sulfate, 100mM Bis-tris-propane pH 7.0, 5% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K