3VO8
Staphylococcus aureus FtsZ GDP-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.039, 44.079, 85.993 |
| Unit cell angles | 96.89, 103.57, 108.00 |
Refinement procedure
| Resolution | 40.113 - 2.255 |
| R-factor | 0.1912 |
| Rwork | 0.188 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vxy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.255 | 2.250 |
| Rmerge | 0.083 | 0.285 |
| Number of reflections | 26863 | |
| Completeness [%] | 97.7 | 91.3 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Lithium sulfate, 0.1M Tris-HCl (pH 8.5), 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
