3VMX
Crystal Structure of a parallel coiled-coil dimerization domain from the voltage-gated proton channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-11-22 |
Detector | RAYONIX MX225HE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.156, 54.010, 81.439 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.010 - 1.450 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.027 |
RMSD bond angle | 2.483 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.056 | 0.440 |
Number of reflections | 232980 | |
<I/σ(I)> | 43.972 | 4.741 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 2% V/V TACSIMATE (PH 7.0), 0.1M IMIDAZOLE (PH 7.0), 8% W/V PEG 3350, 5% V/V 2-PROPANOL (PEGRX-2 # 26, HAMPTOM), VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 283K |