3VKI

Monoclinic Crystal Structure of Salmonella FlgA in closed form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-19
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	53.926, 103.317, 85.501
Unit cell angles	90.00, 107.26, 90.00

Refinement procedure

Resolution	25.000 - 2.300
R-factor	0.2529
Rwork	0.251
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.969
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_3.3.16)
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	39534
Completeness [%]	99.3	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	289	12% PEG 6000, 0.05M CITRIC ACID PH 4.2, 1.0M LICl, 14% 2-METHYL-2,4-PENTANEDIOL, vapor diffusion, hanging drop, temperature 289K