3VK1
Green-fluorescent variant of the non-fluorescent chromoprotein Rtms5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.956600 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 93.120, 93.120, 76.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.620 - 2.200 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.24210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mou |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.981 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.620 | 2.250 | |
| High resolution limit [Å] | 2.200 | 9.820 | 2.200 |
| Rmerge | 0.068 | 0.046 | 0.967 |
| Rmeas | 0.053 | 1.049 | |
| Number of reflections | 17621 | 203 | 1250 |
| <I/σ(I)> | 15.88 | 32.72 | 1.91 |
| Completeness [%] | 98.9 | 80.6 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.36M KI, 0.2M TRIS, PH 8.5, 21% PEG 3350, 25% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
