3VJP
Orthorhombic Crystal Structure of Salmonella FlgA in closed form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97895 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.168, 162.502, 103.485 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.545 - 2.700 |
R-factor | 0.2453 |
Rwork | 0.244 |
R-free | 0.27650 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.044 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (CCP4_3.3.9) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.630 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Total number of observations | 12094 | |
Number of reflections | 12420 | |
Completeness [%] | 98.7 | 99.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289 | 12% PEG 6000, 0.1M CITRIC ACID, 1.0M LiCl, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |