3VH8
KIR3DL1 in complex with HLA-B*5701
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9536 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.248, 66.374, 93.361 |
| Unit cell angles | 100.56, 92.83, 101.56 |
Refinement procedure
| Resolution | 35.850 - 1.800 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2RFX and 1IM9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.221 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.257 | 0.257 |
| Number of reflections | 136837 | |
| <I/σ(I)> | 20.8 | 3 |
| Completeness [%] | 94.6 | 87.8 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 16% PEG 3350, 2% tacsimate pH 5.0, 0.1M tri-sodium citrate pH 5.6., VAPOR DIFFUSION, HANGING DROP, temperature 294K |
