3VGX
Structure of gp41 T21/Cp621-652
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-14 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 44.967, 44.967, 209.237 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.711 - 1.740 |
| R-factor | 0.1923 |
| Rwork | 0.192 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3F4Y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.832 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.711 | 1.800 |
| High resolution limit [Å] | 1.739 | 1.740 |
| Rmerge | 0.034 | 0.179 |
| Number of reflections | 8601 | |
| <I/σ(I)> | 33 | 5.6 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 6.51 | 3.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 295 | 0.05M Tris HCl, 0.05M Potassium, 0.01M Magnesium chloride, 15% (w/v) PEG 6000, pH 8.5, VAPOR DIFFUSION, temperature 295K |
