3VFG
Crystal structure of monoclonal antibody 3F8 Fab fragment that binds to GD2 ganglioside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.028, 64.310, 57.241 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 28.580 - 1.650 |
| R-factor | 0.17842 |
| Rwork | 0.176 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aju |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.094 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.324 | |
| Number of reflections | 49781 | |
| <I/σ(I)> | 3 | |
| Completeness [%] | 98.4 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | Hamptom Index D7, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
