3VEP
Crystal structure of SigD4 in complex with its negative regulator RsdA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.740, 110.720, 73.130 |
| Unit cell angles | 90.00, 133.00, 90.00 |
Refinement procedure
| Resolution | 38.465 - 2.500 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.28660 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.606 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.465 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.068 | 0.442 |
| Number of reflections | 18114 | |
| <I/σ(I)> | 15.6 | 4.4 |
| Completeness [%] | 86.4 | 98 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | OIL-BATCH | 7.4 | 300 | 1.0-103M Ammonium Sulphate, 0.1M HEPES, 15-20% PEG 4000, pH 7.4, OIL-BATCH, temperature 300K |
