3VEA
Crystal Structure of matP-matS23mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.980, 111.310, 169.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.870 - 2.550 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3evb |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.870 | 2.780 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 20349 | |
| Completeness [%] | 88.0 | 58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG400, 0.1 M magnesium chloride, 0.1 M Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
