3VDU
Structure of recombination mediator protein RecRK21G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Wavelength(s) | 0.9875 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 66.848, 123.729, 135.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.724 - 2.800 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vdp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.543 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 6949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 8%(w/v) PEG4000, 200mM Ammonium Sulfate, 10%(v/v) 2-Propanol, 100mM HEPES Sodium Salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
