Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2009-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 63 |
| Unit cell lengths | 83.967, 83.967, 115.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.990 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eoj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.050 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.186 | 0.580 |
| Number of reflections | 29980 | |
| <I/σ(I)> | 1.2 | |
| Completeness [%] | 94.5 | 94 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 mM HEPES, pH 7.5, 16% PEG2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
