3VD6
Both Zn Fingers of GATA1 Bound to Palindromic DNA Recognition Site, P21 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.331, 37.401, 65.484 |
| Unit cell angles | 90.00, 98.51, 90.00 |
Refinement procedure
| Resolution | 14.900 - 1.980 |
| R-factor | 0.2196 |
| Rwork | 0.218 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dfx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 5.300 | 1.980 |
| Rmerge | 0.049 | 0.035 | 0.580 |
| Number of reflections | 18281 | ||
| <I/σ(I)> | 19.1 | ||
| Completeness [%] | 99.5 | 99.9 | 99.9 |
| Redundancy | 3.5 | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M ammonium acetate, 0.1M BISTRIS, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
