3VD5
E. coli (lacZ) beta-galactosidase (N460S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.299, 150.745, 131.086 |
| Unit cell angles | 90.00, 104.06, 90.00 |
Refinement procedure
| Resolution | 54.580 - 2.700 |
| R-factor | 0.20623 |
| Rwork | 0.203 |
| R-free | 0.27571 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3t09 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.126 | 0.311 |
| Number of reflections | 118707 | |
| <I/σ(I)> | 4.23 | 1.03 |
| Completeness [%] | 90.9 | 90.6 |
| Redundancy | 1.96 | 1.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 10% PEG8000, 100 mM Bis-Tris, pH 6.5, 200 mM magnesium chloride, 100 mM sodium chloride, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
