3VA0

Crystal structure of RNase T in complex with a di-nucleotide product (GG) with one Mg in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9999
Spacegroup name	P 21 21 2
Unit cell lengths	95.688, 105.807, 47.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.389 - 2.201
R-factor	0.2089
Rwork	0.206
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ngy
RMSD bond length	0.002
RMSD bond angle	0.588
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	24276
<I/σ(I)>	10.04	2.47
Completeness [%]	97.0	95.2
Redundancy	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.2M Magnesiumformate dihydrate, 0.1M Sodium acetate trihydrate, 18%(w/v) Polyethylene glycol monomethyl ester 5000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K