3V9S
Crystal structure of RNase T in complex with a product ssDNA (AAC) with one Mg in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.063, 105.840, 46.918 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.659 - 2.100 |
| R-factor | 0.2104 |
| Rwork | 0.207 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.487 | |
| Number of reflections | 28735 | |
| <I/σ(I)> | 15.9 | 3.5 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 0.1M Citric acid, 14% PEG1000, 10mM Spermidine, 5mM Magnesium chloride, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
