3V95
Crystal structure of monoclonal human anti-rhesus D Fc and IgG1 t125(yb2/0) in the presence of EDTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.380, 79.120, 138.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.890 - 2.700 |
| R-factor | 0.255 |
| Rwork | 0.251 |
| R-free | 0.32100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3v8c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.795 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.5.0109) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.690 |
| Rmerge | 0.090 | 0.538 |
| Number of reflections | 15731 | |
| <I/σ(I)> | 17.56 | 3.78 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 7.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 293 | 12.8% MPEG 5000, 21.5% ETHYLENE GLYCOL, 0.002M EDTA, 0.1 M SODIUM CACODYLATE, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
