3V95

Crystal structure of monoclonal human anti-rhesus D Fc and IgG1 t125(yb2/0) in the presence of EDTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-05
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	49.380, 79.120, 138.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 2.700
R-factor	0.255
Rwork	0.251
R-free	0.32100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3v8c
RMSD bond length	0.013
RMSD bond angle	1.795
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	REFMAC (5.5.0109)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.700	2.690
Rmerge	0.090	0.538
Number of reflections	15731
<I/σ(I)>	17.56	3.78
Completeness [%]	99.4	97.2
Redundancy	7.8	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.1	293	12.8% MPEG 5000, 21.5% ETHYLENE GLYCOL, 0.002M EDTA, 0.1 M SODIUM CACODYLATE, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K