3V8L

Crystal structure of Staphylococcus aureus biotin protein ligase in complex with biotinyl-5'-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	277
Wavelength(s)	1.518
Spacegroup name	P 42 21 2
Unit cell lengths	93.665, 93.665, 131.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 2.600
R-factor	0.17716
Rwork	0.174
R-free	0.23478
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.826
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.550
High resolution limit [Å]	2.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10% PEG 8000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K