3V8F
Crystal structure of crosslinked GltPh V216C-M385C mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.300, 199.810, 111.251 |
Unit cell angles | 90.00, 117.08, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.800 |
R-factor | 0.244 |
Rwork | 0.243 |
R-free | 0.25457 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.664 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 96.700 |
High resolution limit [Å] | 3.800 |
Number of reflections | 46875 |
Completeness [%] | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 15 - 20% PEG 350 MME, 0.1 M Hepes, ph = 7.4, 0.15 - 0.3 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |