3V7S

Crystal structure of Staphylococcus aureus biotin protein ligase in complex with inhibitor 0364

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	277
Collection date	2009-09-19
Wavelength(s)	1.51
Spacegroup name	P 42 21 2
Unit cell lengths	93.500, 93.500, 130.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.970 - 3.100
R-factor	0.19162
Rwork	0.189
R-free	0.24597
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.752
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	58.970
High resolution limit [Å]	3.100
Number of reflections	19899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10% PEG 8000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K