3V6T
Crystal structure of the DNA-bound dHax3, a TAL effector, at 1.85 angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.719, 87.679, 88.494 |
Unit cell angles | 90.00, 103.04, 90.00 |
Refinement procedure
Resolution | 32.316 - 1.850 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.22170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v6p |
RMSD bond length | 0.008 |
RMSD bond angle | 1.157 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 103239 | |
Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 8-10% PEG 3350(w/v), 12% ethanol, 0.1M MES pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |