3V61

Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	I 41
Unit cell lengths	140.060, 140.060, 52.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2163
Rwork	0.214
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1PLQ and 1EUV
RMSD bond length	0.009
RMSD bond angle	1.289
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.080	0.044	0.376
Number of reflections	23649
<I/σ(I)>	10
Completeness [%]	97.2	99.3	89.4
Redundancy	3.1	3.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	21% MPD, 100 mM BaCl2, 100 mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K