3V5Z

Structure of FBXL5 hemerythrin domain, C2 cell, grown anaerobically

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-19
Detector	ADSC Q315
Wavelength(s)	0.97929
Spacegroup name	C 1 2 1
Unit cell lengths	60.442, 76.051, 77.433
Unit cell angles	90.00, 112.12, 90.00

Refinement procedure

Resolution	33.830 - 2.185
R-factor	0.1794
Rwork	0.177
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v5x
RMSD bond length	0.008
RMSD bond angle	1.012
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.184	5.970	2.184
Rmerge	0.062	0.039	0.552
Number of reflections	16703
<I/σ(I)>	14.9
Completeness [%]	99.7	95	100
Redundancy	4	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	12% PEG 6000, 0.1 M HEPES, 30% ethylene glycol, 2M Na(2)S(2)O(4), pH 6.5, vapor diffusion, hanging drop, temperature 293K