3V5Z
Structure of FBXL5 hemerythrin domain, C2 cell, grown anaerobically
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-19 |
| Detector | ADSC Q315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.442, 76.051, 77.433 |
| Unit cell angles | 90.00, 112.12, 90.00 |
Refinement procedure
| Resolution | 33.830 - 2.185 |
| R-factor | 0.1794 |
| Rwork | 0.177 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v5x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.012 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.184 | 5.970 | 2.184 |
| Rmerge | 0.062 | 0.039 | 0.552 |
| Number of reflections | 16703 | ||
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 99.7 | 95 | 100 |
| Redundancy | 4 | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 12% PEG 6000, 0.1 M HEPES, 30% ethylene glycol, 2M Na(2)S(2)O(4), pH 6.5, vapor diffusion, hanging drop, temperature 293K |
