3V5R

Crystal structure of the unliganded form of Gal3p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 32 2 1
Unit cell lengths	153.359, 153.359, 118.591
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.907 - 2.099
R-factor	0.1713
Rwork	0.170
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	S. cerevisiae Gal1p (pdb code: 2AJ4)
RMSD bond length	0.007
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	132.453	46.919	2.128
High resolution limit [Å]	2.099	6.516	2.099
Number of reflections	93763
Completeness [%]	99.9	99	97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	290	10 mM MgCl2, 50 mM MES, 1.85M Li2SO4, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 290K