3V58
Crystal Structure of the B-phycoerythrin from the red algae Porphyridium Cruentum at pH5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 187.000, 187.000, 59.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lia |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.411 |
| Data reduction software | MOSFLM (3.3.20) |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 93.500 | 19.814 | 1.950 |
| High resolution limit [Å] | 1.850 | 5.850 | 1.850 |
| Rmerge | 0.071 | 0.022 | 0.483 |
| Total number of observations | 9587 | 31314 | |
| Number of reflections | 64691 | ||
| <I/σ(I)> | 13.1 | 26.3 | 1.6 |
| Completeness [%] | 98.0 | 96.5 | 96.7 |
| Redundancy | 3.7 | 4.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | counter-diffusion in capillary | 5 | 298 | 3M ammonium sulphate, 0.1M sodium acetate, pH 5, counter-diffusion in capillary, temperature 298K |
